| Title | Effect of Interaction between Protocatechualdehyde Produced from Streptomyces lincolnensis M-20 and Copper Ions on Antioxidant and Pro-oxidant Activities | ||
| Author | Kyoung-Ja Kim1*, Jae-Hun Lee1, and Yong-Joon Yang2 | ||
| Address | 1Soonchunhyang University, 2Sangmyung University | ||
| Bibliography | Korean Journal of Microbiology, 50(1),22-26, 2014 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2014.4007 | ||
| Key Words | Streptomyces lincolnensis, antioxidant, copper ions, DNA damage, pro-oxidant, protocatechualdehyde | ||
| Abstract | Protocatechualdehyde (PA) is phenolic compound having antioxidative and ntitumor activities. PA was purified from supernatant of Streptomyces lincolnensis M-20. In the presence of copper ion, PA acted as pro-oxidant. The antioxidant activity was assessed with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, and the pro-oxidant effect of PA on DNA damage as pBR322 plasmid DNA-cleaving agents in the presence of Cu(II) ions was investigated. The involvement of reactive oxygen species (ROS) in the DNA damage was confirmed by the inhibition of the DNA breakage by using glutathione (GSH), specific scavenger of ROS. When the increase in ROS reaches a certain level (the toxic threshold), it may trigger cell death. The formation of the PA/Cu(II) chelate complex was confirmed by reaction with ethylenediamine-tetraacetic acid (EDTA), a well-known chelating agent for metal ions, by using UV/Vis spectroscopic analysis. | ||
| Download PDF | 50(1)_04_p.22-26.pdf | ||