| Title | 풋마름병균의 길항세균 Bacillus amyloliquefaciens SKU-78의 대량 배양 조건 확립 | ||
| Author | 김신덕 and 조홍범* | ||
| Address | 서경대학교 화학생명공학과 | ||
| Bibliography | Korean Journal of Microbiology, 50(1),84-86, 2014 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2014.3062 | ||
| Key Words | Bacillus amyloliquefaciens, bacterial wilt, biocontrol agent, media optimization | ||
| Abstract | Mass production of biocontrol agent is an essential step for its commercial use. Media composition and culture conditions for production of Bacillus amyloliquefaciens SKU-78, a potential biocontrol agent against bacterial wilts, were optimized by a flask culture. Low cost media combining nitrogen and carbon sources were tested. Maximum cell growth (>2 × 109 CFU/ml) was obtained in a medium of 5% soy flour combined with 3% corn starch after 24 h cultivation. The optimum initial pH, temperature and shaking speed was 5.5, 30℃ and 150–250 rpm, respectively. Fermentation of SKU-78 was scaled up in 30 L fermenter and the profiles of cell density, pH, dissolved oxygen and spore formation were recorded. After 8 h lag phase, exponential growth occurred and reached at maximum viable cell number (1.2 × 1011 CFU/ml) after 20 h. The SKU-78 strain grown in a low cost medium exhibited the high suppression of bacterial wilts. The results indicate that SKU-78 strain can be produced in a low cost medium and provide a basis for scaling up to industrial level. | ||
| Download PDF | 50(1)_13_p.84-86.pdf | ||