Title |
Metagenomics analysis of methane metabolisms in manure fertilized paddy soil |
Author |
Son G. Nguyen1,2, Cuong Tu Ho3, Ji-Hoon Lee4, and Tatsuya Unno1* |
Address |
1Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University, Jeju 63243, Republic of Korea, 2Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam, 3Institute of Environmental Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam, 4Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju 54896, Republic of Korea |
Bibliography |
Korean Journal of Microbiology, 52(2),157-165, 2016 |
DOI |
http://dx.doi.org/10.7845/kjm.2016.6007
|
Key Words |
metagenomics, methane, methanogens, microbial community, rice |
Abstract |
Under flooded rice fields, methanogens produce methane which comes out through rice stalks, thus rice fields are known as
one of the anthropogenic sources of atmospheric methane. Studies have shown that use of manure increases amount of methane
emission from rice. To investigate mechanisms by which manure boosts methane emission, comparative soil metagenomics between
inorganically (NPK) and pig manure fertilized paddy soils (PIG) were conducted. Results from taxonomy analysis showed that more
abundant methanogens, methanotrophs, methylotrophs, and acetogens were found in PIG than in NPK. In addition, BLAST results
indicated more abundant carbohydrate mabolisetm functional genes in PIG. Among the methane metabolism related genes, PIG sample
showed higher abundance of methyl-coenzyme M reductase (mcrB/mcrD/mcrG) and trimethylamine-corrinoid protein Co-methyltransferase
(mttB) genes. In contrast, genes that down regulate methane emission, such as trimethylamine monooxygenase (tmm) and phosphoserine/
homoserine phosphotransferase (thrH), were observed more in NPK sample. In addition, more methanotrophic genes (pmoB /amoB /
mxaJ ), were found more abundant in PIG sample. Identifying key genes related to methane emission and methane oxidation may provide
fundamental information regarding to mechanisms by which use of manure boosts methane emission from rice. The study presented
here characterized molecular variation in rice paddy, introduced by the use of pig manure. |
Download PDF |
52(2)_04_p.157-165.pdf |