| Title | 단보] Reactive oxygen species-dependent down-regulation of ubiquitin C-terminal hydrolase in Schizosaccharomyces pombe | ||
| Author | Hannah Jo1, Hye-Won Lim2, Hee-Souk Kwon3, Chang-Jin Lim1, Kwang Hark Park4, Chang Duck Jin4, and Kyunghoon Kim4* | ||
| Address | 1Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea, 2Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea, 3Hankook Cosmo Co., Cheonan 31214, Republic of Korea, 4Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 52(2),236-241, 2016 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2016.6025 | ||
| Key Words | sdu1+, Schizosaccharomyces pombe, deubiquitinating enzyme, reactive oxygen species, ubiquitin C-terminal hydrolase | ||
| Abstract | The Schizosaccharomyces pombe sdu1 + gene, belonging to the PPPDE superfamily of deubiquitinating enzyme (DUB) genes, was previously shown to encode a protein with ubiquitin C-terminal hydrolase (UCH) activity and to participate in the response against oxidative and nitrosative stresses. This work focused on the reactive oxygen species (ROS)-dependent regulation of the S. pombe sdu1 + gene. UCH activities, encoded by the sdu1 + gene, were attenuated in the S. pombe cells exposed to H2O2, superoxide radical-generating menadione (MD), and nitric oxide (NO)-generating sodium nitroprusside (SNP). Reduced glutathione (GSH) and its precursor N-acetylcysteine (NAC) were able to significantly enhance the UCH activities in the absence or presence of H2O2. However, the influences of both GSH and NAC on the ROS levels in the absence or presence of H2O2 were opposite to their effects on the UCH activities under the same conditions. The UCH activities in the Sdu1-overexpressing S. pombe cells were also diminished under exposure to H2O2, MD and SNP, but still remained to be higher than those in the vector control cells. In brief, it is proposed that the S. pombe sdu1 + gene is regulated by ROS in a negative manner, the meaning of which largely remains elusive. | ||
| Download PDF | 52(2)_14_p.236-241.pdf | ||